RESEARCH ARTICLE
Comparative Profiling of mRNA and microRNA Expression in Human Mesenchymal Stem Cells Derived from Adult Adipose and Lipoma Tissues
Li-Pin Kao1, Sung-Liang Yu2, Sher Singh3, Kai-Hung Wang1, An-Pei Kao1, Steven Shoei-Lung Li*, 1, 4
Article Information
Identifiers and Pagination:
Year: 2009Volume: 1
First Page: 1
Last Page: 9
Publisher Id: TOSCJ-1-1
DOI: 10.2174/1876893800901010001
Article History:
Received Date: 31/10/2008Revision Received Date: 18/11/2008
Acceptance Date: 1/12/2008
Electronic publication date: 21/1/2009
Collection year: 2007
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Human mesenchymal stem cells have been isolated from adult adipose tissue and lipoma that is a benign neoplasm of normal fat cells. The lipoma-derived LM6 cells exhibited a higher cumulative population doubling levels as compared with the adipose-derived AM3 cells. The expression profiles of both mRNAs and microRNAs (miRNAs) from AM3 and LM6 cells were found to exhibit considerable similarities except that miRNAs miR-99a and miR-152 were abundantly expressed in AM3, but absent in LM6 cells. The abundantly differentially expressed genes HAS2, VNN1, SLC16A6 and COL11A1 in LM6 cells were shown to be targets of miRNAs miR-99a and/or miR-152. These highly upregulated miRNA target genes, as well as several other abundantly differentially expressed genes such as sushi domain containing 2, keratin associated proteins and tumor necrosis factor family, may explain a higher proliferation potential in LM6 cells compared with AM3 cells