RESEARCH ARTICLE


Multilineage Differentiation Potential of Bone and Cartilage Cells Derived from Explant Culture



Zareen Yameen1, David Leavesley 1, Zee Upton 1, Yin Xiao *, 2
1 Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove Campus, Brisbane, Qld 4059 Australia.
2 Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Australia

Article Information


Identifiers and Pagination:

Year: 2009
Volume: 1
First Page: 10
Last Page: 19
Publisher Id: TOSCJ-1-10
DOI: 10.2174/1876893800901010010

Article History:

Received Date: 10/11/2008
Revision Received Date: 9/12/2008
Acceptance Date: 12/12/2008
Electronic publication date: 15/1/2009
Collection year: 2007

© Xiao et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Institute of Health and Biomedical Innovation, Queensland University of Technology, Kelvin Grove Campus, Brisbane, Qld 4059 Australia.E-mail: yin.xiao@qut.edu.au


Abstract

To date, mesenchymal stem cells (MSCs) from various tissues have been reported, but the yield and differentiation potential of different tissue-derived MSCs is still not clear. This study was undertaken in an attempt to investigate the multilineage stem cell potential of bone and cartilage explant cultures in comparison with bone marrow derived mesenchymal stem cells (BMSCs). The results showed that the surface antigen expression of tissue-derived cells was consistent with that of mesenchymal stem cells, such as lacking the hematopoietic and common leukocyte markers (CD34, CD45) while expressing markers related to adhesion (CD29, CD166) and stem cells (CD90, CD105). The tissue-derived cells were able to differentiate into osteoblast, chondrocyte and adipocyte lineage pathways when stimulated in the appropriate differentiating conditions. However, compared with BMSCs, tissue-derived cells showed less capacity for multilineage differentiation when the level of differentiation was assessed in monolayer culture by analysing the expression of tissuespecific genes by reverse transcription polymerase chain reaction (RT-PCR) and histology. In high density pellet cultures, tissue-derived cells were able to differentiate into chondrocytes, expressing chondrocyte markers such as proteoglycans, type II collagen and aggrecan. Taken together, these results indicate that cells derived from tissue explant cultures reserved certain degree of differentiation properties of MSCs .

Keywords: Mesenchymal stem cells, cell differentiation, plasticity, multipotent, osteoblast, chondrocyte, adipocyte.