RESEARCH ARTICLE


Effects of Culturing on the Stability of the Putative Murine Adipose Derived Stem Cells Markers



Jacquelyn R. Maddox 1, 2, Xinbo Liao1, Feng Li , Christopher Niyibizi *, 1
Penn State College of Medicine, Department of Orthopaedics and Rehabilitation, H089, 500 University Drive, Hershey PA 17033, USA.

Article Information


Identifiers and Pagination:

Year: 2009
Volume: 1
First Page: 54
Last Page: 61
Publisher Id: TOSCJ-1-54
DOI: 10.2174/1876893800901010054

Article History:

Received Date: 11/5/2009
Revision Received Date: 29/5/2008
Acceptance Date: 4/6/2009
Electronic publication date: 17/7/2009
Collection year: 2009

© Niyibizi et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Penn State College of Medicine, Department of Orthopaedics and Rehabilitation, H089, 500 University Drive, Hershey PA 17033, USA. E-mail: cniyibizi@psu.edu


Abstract

Mesenchymal stem cells have generated much interest because of their potential use in regenerative medicine. The major draw back in the application of these cells is that there is no single marker or markers that have been established to identify and aid in isolating the cells from a variety of other cell types. The commonly expressed mesenchymal stem cell surface antigens include CD44, CD73, CD90.2, CD105, and CD146. In the present study we examined the stability of these surface antigens in culture and their potential application in identifying and isolating murine derived adipose derived stem cells. The data showed that the expression of these markers increased with culturing and appeared to stabilize by passage 8; the cells were sorted positively for the surface markers at this passage. Each subset was maintained in culture and evaluated for differentiation toward osteogenic lineage and . The CD73 and CD105 positive cell subsets demonstrated robust differentiation toward osteogenic lineage ; the CD90.2+ cell subset exhibited the least differentiation toward osteogenic lineage. Assessment of the cell subpopulations for in vivo differentiation demonstrated that all the cell subsets exhibited potential to differentiate into osteoblasts. Taken together, these data suggest that this panel of markers although useful in identifying cells with potential to differentiate toward osteogenic lineage, cannot prospectively be used for enriching for ADSC from a variety of other cell types