Upregulation of Adhesion Molecules Sustains Matrix-Free Growth of Human Embryonic Stem Cells



Narmin Bigdeli1, *, Giuseppe Maria de Peppo3, 4, Camilla Karlsson3, 4, Maria Lennerås3, 4, 5, Raimund Strehl6, Johan Hyllner7, Anders Lindahl1, 3
1 Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska University Hospital, Gothenburg University, Gothenburg, Sweden
2 Biomatcell VINN Excellence Center of Biomaterials and Cell Therapy, Göteborg, Sweden
3 Department of Biomaterials, Sahlgrenska Academy at University of Gothenburg, Sweden
4 Tataa Biocenter AB, Göteborg, Sweden
5 Verigraft AB, Arvid Wallgrens Backe 20, 413 46 Gothenburg, Sweden
6 Division of Biotechnology/IFM, Linköping University, 581 83 Linköping, Sweden

Abstract

Background:

Despite recent advances in culture techniques for undifferentiated human embryonic stem cells (hESCs), further improvements are required to facilitate research and translation of these cells in clinical settings. We have previously derived hESC lines that can be cultured in their undifferentiated state on regular plastic culture dishes, without the need for feeder cells or other coating supports, denoted matrix-free growth hESCs (MFG-hESCs).

Objective:

In this study, we further characterize and compare MFG-hESCs to hESCs in order to understand the molecular differences responsible for the unique ability of MFG-hESCs.

Results:

Microarray analysis demonstrated that MFG-hESCs highly resemble feeder-cultured hESCs in global gene expression profile. Two identified groups of genes with differential expression were those encoding for ribosomal proteins and attachment proteins, such as the RGD (Arg-Gly-Asp)-associated proteins. Real-time PCR and flow cytometry corroborated the microarray results. Culture of MFG-hESCs in the presence of RGD peptides resulted in decreased attachment ability compared to cells cultured in the presence of RGES (Arg-Gly-Asp-Ser) peptides.

Conclusion:

This study demonstrates that MFG-hESC lines overexpress cell attachment proteins but retain the typical characteristics of undifferentiated feeder-cultured hESCs. The ability to culture high-quality pluripotent stem cells in feeder- and matrix-free conditions creates a new opportunities for their large-scale manufacturing for experimental research and translational applications.

Keywords: Attachment proteins, Cell therapy, Human embryonic stem cells, Integrins, Matrix-free culture, Microarray analysis, Regenerative medicine, RGD-associated proteins.


Abstract Information


Identifiers and Pagination:

Year: 2018
Volume: 5
Publisher Item Identifier: EA-TOSCJ-2018-5

Article History:

Received Date: 4/9/2018
Revision Received Date: 11/10/2018
Acceptance Date: 16/10/2018
Electronic publication date: 29/10/2018

© 2018 Bigdeli et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Correspondence: Address correspondence to this author at the Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, Sahlgrenska University Hospital, Gothenburg University, Gothenburg, Sweden, Tel: +46739625242; E-mailS: narmin.bigdeli@medic.gu.se; narmin.bigdeli@gu.se
* Narmin Bidgeli and Giuseppe Maria de Peppo contributed equally to the study and both must be considered first author.